A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant role in marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two added splice variants were being reported but comprehensive nucleotide sequence details is not really yet obtainable.
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Blocking peptides are peptides that bind exclusively towards the target antibody and block antibody binding. These peptide usually incorporates the epitope regarded with the antibody.
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This guideline outlines more probable optimization of cellular and immunoassay parameters to make certain the absolute best success are...
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to make sure the very best outcomes are...
Antibodies sure to the blocking peptide now not bind for the epitope about the concentrate on protein. By evaluating the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is unique.
Target Details Cyclin E belongs on the remarkably conserved cyclin family, whose users are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation get more info patterns which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E varieties a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S period boundary and it is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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